UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media

The physicochemical characterization of nanomaterials (NMs) is often an analytical challenge, due to their small size (at least one dimension in the nanoscale, i.e. 1–100 nm), dynamic nature, and diverse properties. At same time, reliable repeatable paramount ensure safety quality manufacturing NM-bearing products. There are several methods available monitor achieve measurement nanoscale-related properties, example which Ultraviolet-Visible Spectroscopy (UV-Vis). This a well-established, simple, inexpensive technique that provides non-invasive fast real-time screening evaluation NM size, concentration, aggregation state. Such features make UV-Vis ideal methodology assess proficiency testing schemes (PTS) validated standard operating procedure (SOP) intended evaluate performance reproducibility method. In this paper, PTS six partner laboratories from H2020 project ACEnano were assessed through interlaboratory comparison (ILC). Standard gold (Au) colloid suspensions different sizes (ranging 5–100 nm) characterized by at institutions develop implementable robust protocol for characterization.